BRAF

 

Full name: B-Raf proto-oncogene, serine/threonine kinase Alias Symbol: BRAF1
Type: protein-coding gene Cytoband: 7q34
Entrez ID: 673 HGNC ID: HGNC:1097 Ensembl Gene: ENSG00000157764 OMIM ID: 164757
Drug and gene relationship at DGIdb
Screen Evidence:
  • Knocking out of this gene by CRIPR/Cas9 screen found 18/325 cell lines had fitness change in Project Score.
  • This gene has been idenfitied as a common driver in this paper.
  • Knocking out of this gene by CRIPR/Cas9 screen found altered tolerance on DNA toxicity in Genotoxic Screens.

 

BRAF involved pathways:

KEGG pathway Description View
hsa04010MAPK signaling pathway
hsa04012ErbB signaling pathway
hsa04015Rap1 signaling pathway
hsa04024cAMP signaling pathway
hsa04062Chemokine signaling pathway
hsa04068FoxO signaling pathway
hsa04150mTOR signaling pathway
hsa04270Vascular smooth muscle contraction
hsa04510Focal adhesion
hsa04650Natural killer cell mediated cytotoxicity
hsa04720Long-term potentiation
hsa04722Neurotrophin signaling pathway
hsa04726Serotonergic synapse
hsa04730Long-term depression
hsa04810Regulation of actin cytoskeleton
hsa04910Insulin signaling pathway
hsa04914Progesterone-mediated oocyte maturation
hsa05034Alcoholism
hsa05160Hepatitis C
hsa05200Pathways in cancer
hsa05205Proteoglycans in cancer
hsa05210Colorectal cancer
hsa05211Renal cell carcinoma
hsa05212Pancreatic cancer
hsa05213Endometrial cancer
hsa05214Glioma
hsa05215Prostate cancer
hsa05216Thyroid cancer
hsa05218Melanoma
hsa05219Bladder cancer
hsa05220Chronic myeloid leukemia
hsa05221Acute myeloid leukemia
hsa05223Non-small cell lung cancer

 

Expression of BRAF:

 

Dataset Gene EntrezID Probe Log2FC Adj.pValue Expression
GSE17351 BRAF 673 226391_at -0.4166 0.2807
GSE26886 BRAF 673 226391_at -0.7646 0.0081
GSE45670 BRAF 673 226391_at -0.5127 0.0012
GSE53622 BRAF 673 51767 -0.5198 0.0000
GSE53624 BRAF 673 51767 -0.4615 0.0000
GSE63941 BRAF 673 226391_at -0.3282 0.4742
GSE77861 BRAF 673 226391_at -0.1087 0.6738
GSE97050 BRAF 673 A_23_P42935 -0.2491 0.3475
SRP007169 BRAF 673 RNAseq -0.0709 0.8498
SRP008496 BRAF 673 RNAseq 0.0058 0.9787
SRP064894 BRAF 673 RNAseq -0.3632 0.0209
SRP133303 BRAF 673 RNAseq -0.1212 0.2785
SRP159526 BRAF 673 RNAseq -0.2537 0.3046
SRP193095 BRAF 673 RNAseq -0.2946 0.0015
SRP219564 BRAF 673 RNAseq -0.4107 0.2551
TCGA BRAF 673 RNAseq -0.0596 0.3837

Upregulated datasets: 0; Downregulated datasets: 0.  

 

Survival by BRAF expression:

 

GSE53622GSE53624TCGA

 

Note: Click image to view full size file.  

Copy number change of BRAF:

 

Dataset Gene EntrezID Gain Loss Normal Detail
GSE15526 BRAF 673 5 2 23
GSE20123 BRAF 673 5 2 23
GSE43470 BRAF 673 2 4 37
GSE46452 BRAF 673 7 2 50
GSE47630 BRAF 673 7 4 29
GSE54993 BRAF 673 3 5 62
GSE54994 BRAF 673 5 8 40
GSE60625 BRAF 673 0 0 11
GSE74703 BRAF 673 2 4 30
GSE74704 BRAF 673 1 2 17
TCGA BRAF 673 28 24 44

Total number of gains: 65; Total number of losses: 57; Total Number of normals: 366.  

 

Somatic mutations of BRAF:

 

Generating mutation plots.

 

Highly correlated genes for BRAF:

 

Showing top 20/185 corelated genes with mean PCC>0.5.
Gene1 Gene2 Mean PCC Num. Datasets Num. PCC<0 Num. PCC>0.5
BRAFRIPK40.784675303
BRAFGPR1460.765617303
BRAFCLN30.761862303
BRAFBCDIN3D0.722983303
BRAFGIGYF20.709027303
BRAFKMT2C0.690827505
BRAFCLEC4D0.685018303
BRAFWNT2B0.679244403
BRAFSEC14L10.676824403
BRAFZNF780A0.674393403
BRAFRUNDC3B0.669628303
BRAFCCSER20.662753404
BRAFADAMTS170.662578403
BRAFEPB41L4A0.654469303
BRAFFAM189A20.653352604
BRAFFAM163B0.653343303
BRAFFHIT0.652535604
BRAFFGF140.6516604
BRAFACKR10.650093303
BRAFCCDC220.649529303

For details and further investigation, click here