RAF1

 

Full name: Raf-1 proto-oncogene, serine/threonine kinase Alias Symbol: Raf-1|c-Raf|CRAF
Type: protein-coding gene Cytoband: 3p25.2
Entrez ID: 5894 HGNC ID: HGNC:9829 Ensembl Gene: ENSG00000132155 OMIM ID: 164760
Drug and gene relationship at DGIdb
Screen Evidence:
  • Knocking out of this gene by CRIPR/Cas9 screen found 17/325 cell lines had fitness change in Project Score.
  • This gene has been idenfitied as a common driver in this paper.
  • Knocking out of this gene by CRIPR/Cas9 screen found altered tolerance on DNA toxicity in Genotoxic Screens.

 

RAF1 involved pathways:

KEGG pathway Description View
hsa04010MAPK signaling pathway
hsa04012ErbB signaling pathway
hsa04014Ras signaling pathway
hsa04015Rap1 signaling pathway
hsa04022cGMP-PKG signaling pathway
hsa04024cAMP signaling pathway
hsa04062Chemokine signaling pathway
hsa04068FoxO signaling pathway
hsa04071Sphingolipid signaling pathway
hsa04072Phospholipase D signaling pathway
hsa04150mTOR signaling pathway
hsa04151PI3K-Akt signaling pathway
hsa04210Apoptosis
hsa04270Vascular smooth muscle contraction
hsa04360Axon guidance
hsa04370VEGF signaling pathway
hsa04510Focal adhesion
hsa04540Gap junction
hsa04550Signaling pathways regulating pluripotency of stem cells
hsa04630Jak-STAT signaling pathway
hsa04650Natural killer cell mediated cytotoxicity
hsa04660T cell receptor signaling pathway
hsa04662B cell receptor signaling pathway
hsa04664Fc epsilon RI signaling pathway
hsa04666Fc gamma R-mediated phagocytosis
hsa04720Long-term potentiation
hsa04722Neurotrophin signaling pathway
hsa04726Serotonergic synapse
hsa04730Long-term depression
hsa04810Regulation of actin cytoskeleton
hsa04910Insulin signaling pathway
hsa04912GnRH signaling pathway
hsa04914Progesterone-mediated oocyte maturation
hsa04915Estrogen signaling pathway
hsa04916Melanogenesis
hsa04917Prolactin signaling pathway
hsa04919Thyroid hormone signaling pathway
hsa04921Oxytocin signaling pathway
hsa05034Alcoholism
hsa05152Tuberculosis
hsa05160Hepatitis C
hsa05161Hepatitis B
hsa05164Influenza A
hsa05200Pathways in cancer
hsa05205Proteoglycans in cancer
hsa05210Colorectal cancer
hsa05211Renal cell carcinoma
hsa05212Pancreatic cancer
hsa05213Endometrial cancer
hsa05214Glioma
hsa05215Prostate cancer
hsa05218Melanoma
hsa05219Bladder cancer
hsa05220Chronic myeloid leukemia
hsa05221Acute myeloid leukemia
hsa05223Non-small cell lung cancer
hsa05231Choline metabolism in cancer

 

Expression of RAF1:

 

Dataset Gene EntrezID Probe Log2FC Adj.pValue Expression
GSE17351 RAF1 5894 201244_s_at -0.1722 0.4672
GSE20347 RAF1 5894 201244_s_at -0.5688 0.0004
GSE23400 RAF1 5894 201244_s_at -0.4574 0.0000
GSE26886 RAF1 5894 201244_s_at -1.2423 0.0000
GSE29001 RAF1 5894 201244_s_at -0.6629 0.0227
GSE38129 RAF1 5894 201244_s_at -0.5669 0.0000
GSE45670 RAF1 5894 201244_s_at -0.3891 0.0018
GSE53622 RAF1 5894 7226 -0.6345 0.0000
GSE53624 RAF1 5894 7226 -0.7190 0.0000
GSE63941 RAF1 5894 201244_s_at -0.4243 0.2679
GSE77861 RAF1 5894 201244_s_at -0.6634 0.0040
GSE97050 RAF1 5894 A_23_P40952 -0.2653 0.2571
SRP007169 RAF1 5894 RNAseq -1.7869 0.0000
SRP008496 RAF1 5894 RNAseq -1.7398 0.0000
SRP064894 RAF1 5894 RNAseq -0.8546 0.0000
SRP133303 RAF1 5894 RNAseq -0.4317 0.0036
SRP159526 RAF1 5894 RNAseq -0.6531 0.0003
SRP193095 RAF1 5894 RNAseq -0.6702 0.0000
SRP219564 RAF1 5894 RNAseq -0.7349 0.0072
TCGA RAF1 5894 RNAseq -0.2545 0.0000

Upregulated datasets: 0; Downregulated datasets: 3.  

 

Survival by RAF1 expression:

 

GSE53622GSE53624TCGA

 

Note: Click image to view full size file.  

Copy number change of RAF1:

 

Dataset Gene EntrezID Gain Loss Normal Detail
GSE15526 RAF1 5894 1 17 12
GSE20123 RAF1 5894 1 18 11
GSE43470 RAF1 5894 0 21 22
GSE46452 RAF1 5894 2 17 40
GSE47630 RAF1 5894 2 23 15
GSE54993 RAF1 5894 6 1 63
GSE54994 RAF1 5894 1 33 19
GSE60625 RAF1 5894 5 0 6
GSE74703 RAF1 5894 0 17 19
GSE74704 RAF1 5894 1 10 9
TCGA RAF1 5894 1 68 27

Total number of gains: 20; Total number of losses: 225; Total Number of normals: 243.  

 

Somatic mutations of RAF1:

 

Generating mutation plots.

 

Highly correlated genes for RAF1:

 

Showing top 20/1397 corelated genes with mean PCC>0.5.
Gene1 Gene2 Mean PCC Num. Datasets Num. PCC<0 Num. PCC>0.5
RAF1SPNS20.831129303
RAF1VSIG20.778709606
RAF1MKRN20.77531611011
RAF1EMC30.77501110010
RAF1FAM3D0.753043606
RAF1C5orf66-AS10.749798606
RAF1CLEC3B0.746221303
RAF1RAB5B0.7452871009
RAF1SMIM50.743499606
RAF1KRT780.740165606
RAF1GPD1L0.73949812011
RAF1SHROOM30.732312707
RAF1CAPNS20.731857404
RAF1CGNL10.730989706
RAF1CYP21A20.730802303
RAF1MAGI30.729543606
RAF1CCDC120.728032606
RAF1ZNF1750.726834303
RAF1FAM214A0.726664605
RAF1GBP60.726551404

For details and further investigation, click here